Figure 1.
Figure 1. Structure of retroviral constructs. (A) The empty vectors pFB-neo and pFB-IRES-GFP were used in experiments as controls for retrovirus infection. The gene inserts were placed behind the 5′ long terminal repeat (LTR) and upstream of the internal ribosomal entry site (IRES), whereas the marker gene (either neo or GFP) was controlled by the IRES. Schematic diagram of wild-type (wt) and chimeric (ch) proteins are shown in panel B. The extracellular (EC), transmembrane (TM), and cytoplasmic regions (CYP) are indicated. In the chNKG2D, the CD3ζ chain was fused to the N-terminus of the NKG2D molecule in a reverse (Rev) orientation (COOH-terminus > NH2-terminus). In chDap10, the CD3ζ chain was placed downstream of the COOH-terminus of the Dap10 molecule in a normal orientation.

Structure of retroviral constructs. (A) The empty vectors pFB-neo and pFB-IRES-GFP were used in experiments as controls for retrovirus infection. The gene inserts were placed behind the 5′ long terminal repeat (LTR) and upstream of the internal ribosomal entry site (IRES), whereas the marker gene (either neo or GFP) was controlled by the IRES. Schematic diagram of wild-type (wt) and chimeric (ch) proteins are shown in panel B. The extracellular (EC), transmembrane (TM), and cytoplasmic regions (CYP) are indicated. In the chNKG2D, the CD3ζ chain was fused to the N-terminus of the NKG2D molecule in a reverse (Rev) orientation (COOH-terminus > NH2-terminus). In chDap10, the CD3ζ chain was placed downstream of the COOH-terminus of the Dap10 molecule in a normal orientation.

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