chNKG2D and chDap10 molecules express as efficiently as their wild-type counterparts on cell surface. (A) NKG2D expression on Bosc23 cells was evaluated 24 hours after transfection by combination of plasmids containing NKG2D and adaptor genes. Surface expression of NKG2D was determined on the gated GFP⁺ population using the PE-conjugated anti-NKG2D Ab (unshaded region). Isotype controls are shown in shaded region. The percentage of NKG2D⁺ cells is indicated. (B) NKG2D expression on B6 T cells 7 days after transduction. NKG2D expression was measured using anti-NKG2D mAbs in combination with anti-CD4 and anti-CD3 mAbs. More than 97% cells are CD3⁺ T cells (data not shown). CD4^– T cells are CD8⁺ T cells. The dot plots shown were all gated on CD3⁺ cells. The percentage of cells in each quadrant is indicated. The data are a representative of 6 similar experiments.