Lytic activity of chNKG2D-bearing T cells is dependent upon NKG2D recognition but not target cell MHC expression. Effector T cells modified with vector only (A,C) or chNKG2D (B,D) were cocultured with target cells RMA/Rae-1β (A-B) or EG7 (C-D) in the presence of anti-NKG2D antibodies (▪) or control antibodies (□), and percentage of specific lysis determined after a 4-hour ⁵¹Cr release assay. Effector T cells modified with vector only (○) or chNKG2D (•) were cocultured with RMA-S (E), or RMA-S/Rae-1β (F) cells, respectively, in 4-hour ⁵¹Cr release assays. The data are presented as means ± SD and are representative of 2 or 3 independent experiments.