American Society of Hematology

Figure 5.

$Figure 5. Migrating DCs after ApoC/TNF-α or PGE2/TNF-α stimuli are enriched in mature cells. DCs were exposed to apoptotic Jurkat cells at an increasing DC/ApoC ratio before the addition of TNF-α. (A) The percentage of mature DCs was measured by CD83 expression before (total DCs) or after (migrating DCs) migration toward CCL21. (B) Total or migrating DCs were mixed at a 1:40 ratio with allogeneic T cells intracellularly stained with CFSE. At day 3, T cells were analyzed using flow cytometry. Percentages represent the fraction of proliferating cells in which CFSE was diluted at least 2-fold after 3 days.$

Migrating DCs after ApoC/TNF-α or PGE2/TNF-α stimuli are enriched in mature cells. DCs were exposed to apoptotic Jurkat cells at an increasing DC/ApoC ratio before the addition of TNF-α. (A) The percentage of mature DCs was measured by CD83 expression before (total DCs) or after (migrating DCs) migration toward CCL21. (B) Total or migrating DCs were mixed at a 1:40 ratio with allogeneic T cells intracellularly stained with CFSE. At day 3, T cells were analyzed using flow cytometry. Percentages represent the fraction of proliferating cells in which CFSE was diluted at least 2-fold after 3 days.

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