ApoC plus LPS allows the migration of DCs that polarize naive T cells toward TH1 response. DCs exposed to apoptotic Jurkat cells (DC/ApoC ratio of 1:8) or PGE2 (3.5 μg/mL) were incubated for 2 days with TNF-α (250 U/mL) or LPS (5 μg/mL). DCs migrating toward CCL21 were mixed in X-VIVO media at a 1:40 ratio with allogeneic naive CD4 T cells. Between day 10 and day 13, T cells were harvested, stained intracellularly for IFN-γ or IL-4, and analyzed by flow cytometry. (A) Percentage of T cells positive for intracellular IFN-γ (mean ± SD of 3 experiments) and FACS histograms of a representative experiment. (B) Percentage of T cells positive for intracellular IL-4 (mean ± SD of 2 experiments) and FACS histograms of a representative experiment. Percentages of IFN-γ- or IL-4-positive cells as defined using isotype control are given.
