American Society of Hematology

Figure 3.

$Figure 3. T140, TC14012, and TN14003 inhibit CXCL12-induced p42/44 MAPK activation and STAT3 serine phosphorylation. Cells were preincubated with 100 μg/mL T140, TC14012, and TN14003 for 30 minutes before stimulation with 200 ng/mL CXCL12 for 10 minutes. (A) Western blot analysis was performed on cell lysates with anti-phospho-p42/44 (top blot) and anti-p42/44 (bottom blot) antibodies. The blot shows representative results from 1 of 5 experiments with CLL B cells from different patients. (B) Western blot analysis was performed on cytosolic fractions with an antibody specific for serine phosphorylation of STAT3 (top blot). The bottom blot shows the membrane reprobed with an anti-STAT3 antibody. (C) Densitometry of the Western blots confirmed the reduction in p42/44 MAPK and STAT3 activation by preincubation with CXCR4 antagonists. Bars represent the phosphorylation of p42/44 MAPK and STAT3, as displayed in panels A and B after analysis by densitometry relative to the unstimulated controls (left-hand block).$

T140, TC14012, and TN14003 inhibit CXCL12-induced p42/44 MAPK activation and STAT3 serine phosphorylation. Cells were preincubated with 100 μg/mL T140, TC14012, and TN14003 for 30 minutes before stimulation with 200 ng/mL CXCL12 for 10 minutes. (A) Western blot analysis was performed on cell lysates with anti-phospho-p42/44 (top blot) and anti-p42/44 (bottom blot) antibodies. The blot shows representative results from 1 of 5 experiments with CLL B cells from different patients. (B) Western blot analysis was performed on cytosolic fractions with an antibody specific for serine phosphorylation of STAT3 (top blot). The bottom blot shows the membrane reprobed with an anti-STAT3 antibody. (C) Densitometry of the Western blots confirmed the reduction in p42/44 MAPK and STAT3 activation by preincubation with CXCR4 antagonists. Bars represent the phosphorylation of p42/44 MAPK and STAT3, as displayed in panels A and B after analysis by densitometry relative to the unstimulated controls (left-hand block).

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