Expression of surface molecules on DCs treated with IFN-γ or IL-4. Expressions of CD1d, CD40, CD86, H-2K^d, and I-A^d on iDCs, IFN/DCs, or IL-4/DCs derived from BC1 cells and SDDCs (WT or CD1d^-/-) were analyzed by flow cytometry. (A) Representative histogram of each molecule on iDCs, IFN/DCs, or IL-4/DCs. Mean fluorescence intensity (MFI) of each histogram is shown at upper right of the each panel. (B) Each column represents the mean ± SE of 3 independent experiments. MFI subtracted from the level of isotype-matched control Ig is shown. Statistical significance was calculated by Student t test (^*P < .05; ^**P < .01 vs iDCs).