The effect of IL-4 or IFN-γ on preference of DCs to induce Th differentiation. DO11.10 CD4⁺ T cells were cocultured with iDCs, IL-4/DCs, or IFN/DCs from BC1 cells in the presence of a high- or low-dose OVA_323-339 peptide for 5 days. The cells were stained by KJ1-26 mAb and monitored for intracellular staining of IFN-γ and IL-4. (A) Dot plots gated by KJ1-26⁺ cells. Results are representative of 3 independent experiments. (B) Proportions of IL-4⁺IFN-γ^- (Th2, left side) or IFN-γ⁺IL-4^- (Th1, right side) cells in the KJ1-26⁺ population. Each column represents the mean ± SE of 3 independent experiments. Statistical significance was calculated by Student t test (^*P < .05 vs iDCs).