Conditional depletion of PU.1 in adult hematopoiesis. FACS analysis of Mx1-Cre × PU.1–/F mice 3 (A-B) and 6 (C-D) weeks after pI-pC injection. Three weeks after the injection, HSCs were decreased up to 5-fold, and CMPs and GMPs disappeared (A). At this time point, the vast majority of bone marrow cells excised loxP-flanked PU.1 alleles (not shown). Significant recovery of these cells was observed at 6 weeks (C), when the excised allele of PU.1 locus was undetectable (not shown). CLPs were eliminated 3 weeks following disruption of PU.1 (B), while they recovered at the 6-week time point (D). Numbers in each panel represent percentages of the gated population in whole bone marrow cells. Summarized data including results of pI-pC injection into Mx1-Cre × PU.1F/F mice are shown in Table 1.
