Figure 2.
Figure 2. Method to identify the proliferative potential of individual endothelial cells (ECs). ECs derived from cord or adult blood EPCs were transduced with a retrovirus-encoding green fluorescence protein, the expressing cells were identified, and single cells were plated using a fluorescence-activated cell sorter equipped with a single-cell deposition device. The green fluorescing cells were visualized through a fluorescence microscope, and the number of cells in each well was directly counted. Microscope, objective, and camera details are as in Figure 1.

Method to identify the proliferative potential of individual endothelial cells (ECs). ECs derived from cord or adult blood EPCs were transduced with a retrovirus-encoding green fluorescence protein, the expressing cells were identified, and single cells were plated using a fluorescence-activated cell sorter equipped with a single-cell deposition device. The green fluorescing cells were visualized through a fluorescence microscope, and the number of cells in each well was directly counted. Microscope, objective, and camera details are as in Figure 1.

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