Expression of PKC isoforms and phosphorylation of PKCμ following Wnt-3a treatment. (A) Cell lysates from the indicated lines were resolved on 8% SDS-PAGE gels, transferred to membranes, and blotted with the indicated antibodies. Lysate from Jurkat cells was included as a control for PKC expression. (B) Myeloma cell lines starved in serum-free medium for 12 hours were treated with Wnt-3a or con-CM for the indicated time. Lysates were resolved on 8% SDS-PAGE gels, transferred to membranes, and blotted with anti-p-PKCμ (B) or anti-p-pan-PKC (C) antibodies.