Figure 1.
Figure 1. Mature mouse mast cells express mouse CD34 but not transgenic human CD34. (A) BMMC cultures were established from wild-type (wt) and hCD34tg bone marrow and assessed for expression of mouse and human CD34 and for c-kit and FcϵRI as markers of mast-lineage cells by flow cytometry. Blue lines indicate wild-type BMMCs; green lines, hCD34tg BMMCs; red lines, secondary antibody alone; and orange lines, human CD34 staining of KG1a cells. (B) Reverse-transcription-polymerase chain reaction (RT-PCR) analysis of BMMCs and KG1a cells for expression of mouse and human CD34 mRNA. Two bands in mouse CD34 lanes correspond to the expected size of the 2 naturally occurring splice variants of mouse CD34 detected by the murine primers.

Mature mouse mast cells express mouse CD34 but not transgenic human CD34. (A) BMMC cultures were established from wild-type (wt) and hCD34tg bone marrow and assessed for expression of mouse and human CD34 and for c-kit and FcϵRI as markers of mast-lineage cells by flow cytometry. Blue lines indicate wild-type BMMCs; green lines, hCD34tg BMMCs; red lines, secondary antibody alone; and orange lines, human CD34 staining of KG1a cells. (B) Reverse-transcription-polymerase chain reaction (RT-PCR) analysis of BMMCs and KG1a cells for expression of mouse and human CD34 mRNA. Two bands in mouse CD34 lanes correspond to the expected size of the 2 naturally occurring splice variants of mouse CD34 detected by the murine primers.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal