HO-1 expression decreases during rat and human DC maturation. (A) Western blot analysis of HO-1 expression in rat immature (IM) BMDCs and following maturation with TNF-α, poly(I:C), CpG, CD40L, and LPS was carried out using an anti-HO-1 antibody. Anti-β tubulin was used as a loading control. Bar graph shows densitometry analysis ± SD of HO-1 signals after normalization with β-tubulin from 3 different experiments. (B) Western blot analysis of HO-1 expression in human immature (IM) monocyte-derived DCs and human DCs following maturation induction with TNF-α, poly(I:C), CD40L, and LPS, performed using an anti-HO-1 antibody. Anti-β tubulin was used as a loading control. Bar graph shows densitometry analysis ± SD of HO-1 signals, after normalization with β-tubulin, from 2 different experiments. (C) Time-course Western blot analysis of HO-1 expression in human immature (IM) monocyte-derived DCs and human DCs following maturation induction with LPS, performed with an anti-HO-1 antibody. Anti-β tubulin was used as a loading control. Bar graph shows densitometry analysis of HO-1 signals after normalization with β-tubulin. (D) Human immature (IM) monocyte-derived DCs were cultured with or without 20 ng/mL of IL-10 for 18 hours and then with or without 1 μg/mL of LPS for 24 hours. Western blot was performed with an anti-HO-1. Anti-β tubulin was used as a loading control. Histograms show densitometry analysis ± SD of HO-1 signals after normalization with β tubulin, from 2 different experiments.