American Society of Hematology

Figure 4.

$Figure 4. Induction of HO-1 renders DCs refractory to LPS-induced phenotypic maturation. (A) Western blot analysis of HO-1 levels in human iDCs treated or not with SnPP or CoPP for 2 hours, cultured for 16 hours, and then for a further 24 hours in the presence or absence of LPS. Anti-β tubulin was used as a loading control. (B) Flow cytometry analysis showing the phenotype of immature (IM) rat BMDCs treated or not with SnPP or CoPP for 2 hours, cultured for 16 hours, and then for a further 24 hours with or without LPS for 24 hours. Expression of MHC class II, CD80, CD86, and intercellular adhesion molecule 1 (ICAM-1) was assessed. Similar results were obtained in 5 independent experiments. Numbers within graph quadrants are percentage of positive cells. (C) Flow cytometry analysis showing the phenotype of immature human DCs treated or not with SnPP or CoPP for 2 hours, cultured for 16 hours, and then stimulated or not with LPS for 24 hours. Thin gray line indicates untreated immature DCs; dashed line, untreated DCs stimulated with LPS; thin black line, treatment with CoPP or SnPP; bold line, treatment with CoPP or SnPP and stimulated with LPS; and dotted line, isotype. Expression of CD83, CD80, CD86, and MHC class I was assessed. Similar results were obtained in 3 independent experiments.$

Induction of HO-1 renders DCs refractory to LPS-induced phenotypic maturation. (A) Western blot analysis of HO-1 levels in human iDCs treated or not with SnPP or CoPP for 2 hours, cultured for 16 hours, and then for a further 24 hours in the presence or absence of LPS. Anti-β tubulin was used as a loading control. (B) Flow cytometry analysis showing the phenotype of immature (IM) rat BMDCs treated or not with SnPP or CoPP for 2 hours, cultured for 16 hours, and then for a further 24 hours with or without LPS for 24 hours. Expression of MHC class II, CD80, CD86, and intercellular adhesion molecule 1 (ICAM-1) was assessed. Similar results were obtained in 5 independent experiments. Numbers within graph quadrants are percentage of positive cells. (C) Flow cytometry analysis showing the phenotype of immature human DCs treated or not with SnPP or CoPP for 2 hours, cultured for 16 hours, and then stimulated or not with LPS for 24 hours. Thin gray line indicates untreated immature DCs; dashed line, untreated DCs stimulated with LPS; thin black line, treatment with CoPP or SnPP; bold line, treatment with CoPP or SnPP and stimulated with LPS; and dotted line, isotype. Expression of CD83, CD80, CD86, and MHC class I was assessed. Similar results were obtained in 3 independent experiments.

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