Figure 5.
Figure 5. HNK modulates growth and survival signaling pathways in MM.1S cells. (A) MM.1S cells were pretreated with HNK (10 μg/mL) in 2.5% FCS containing media for 3 hours and 6 hours; cells were then stimulated with IL-6 (10 ng/mL) for 10 and 20 minutes. Whole-cell lysates were subjected to Western blotting to assess phosphorylation and protein expression of STAT3, ERK2, and Akt. (B) MM.1S cells were pretreated with HNK (10 μg/mL) in 2.5% FCS containing media for 3 hours and 6 hours, and then stimulated with IGF-1 (25 ng/mL) for 10 and 20 minutes. Whole-cell lysates were subjected to Western blotting for phosphorylation and protein expression of ERK2 and Akt. (C) MM.1S cells were pretreated with HNK (10 μg/mL) in 2.5% FCS containing media for 3 hours and 6 hours. Whole-cell lysates were subjected to Western blotting to determine cleavage of caspases and expression of gp80 and gp130.

HNK modulates growth and survival signaling pathways in MM.1S cells. (A) MM.1S cells were pretreated with HNK (10 μg/mL) in 2.5% FCS containing media for 3 hours and 6 hours; cells were then stimulated with IL-6 (10 ng/mL) for 10 and 20 minutes. Whole-cell lysates were subjected to Western blotting to assess phosphorylation and protein expression of STAT3, ERK2, and Akt. (B) MM.1S cells were pretreated with HNK (10 μg/mL) in 2.5% FCS containing media for 3 hours and 6 hours, and then stimulated with IGF-1 (25 ng/mL) for 10 and 20 minutes. Whole-cell lysates were subjected to Western blotting for phosphorylation and protein expression of ERK2 and Akt. (C) MM.1S cells were pretreated with HNK (10 μg/mL) in 2.5% FCS containing media for 3 hours and 6 hours. Whole-cell lysates were subjected to Western blotting to determine cleavage of caspases and expression of gp80 and gp130.

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