Binding of M33-containing PcG complex at Ad4BP/SF1 gene locus. Primer pairs (1 to 17) used for ChIP assay are indicated in the map of the gene locus carrying the last exon of Gcnf, Ad4BP/SF1, and the 20th and 21st exons of a predicted gene, Gpr144. The middle panel summarizes our previous results. DNase I hypersensitive sites (adHS1, adHS2, and adHS3), acetylated histone tails of H3 and H4, nuclear matrix attachment region (MAR), and CCCTC-binding factor (Ctcf)–binding region are distributed as indicated previously.40 Chromatin prepared from adrenocortical Y-1 cells was subjected to immunoprecipitation with anti-M33 antibody (αM33) or control IgG (IgG). The recovered DNAs were amplified with the primers listed in “Materials and methods.” As a control, 0.1% input DNA was amplified (input).
