Overexpression of NF-κB-p65 protects L540cy cells from RAD-induced cell-cycle arrest in G0/G1. (A) L540cy cells were transfected with a p65-GFP expression construct or with control (pcDNA3) and EGFP-expressing (pEGFP-N3) constructs. At 24 hours after transfection, whole cell extracts were analyzed by Western blotting for the expression of p65-GFP and endogenous p65 using a p65-specific antibody. (B) L540cy cells were transfected with a p65-GFP expression construct or with pcDNA3 and pEGFP-N3 constructs. At 6 hours after transfection, cells were incubated with 0 nM and 10 nM RAD for 24 hours. RAD-treated and untreated cells were counted and counts of untreated cells were set arbitrarily at 100%. Cell counts are given as the mean of 3 independent experiments. Error bars indicate SD.
