Secretion and serum levels of sCD95, and CD95 expression. Cell supernatant from proliferating EBV-transformed B cells (A; n = 5) and from PBMCs (B; n = 2) that were activated with PHA for 2 days and IL-2 for 3 days (5 days of total incubation) was collected to determine sCD95 secretion; p, patient; c, healthy donor. (C) CD95 expression by PBMCs after 5 days of activation; representative result, see also Table 3. In the rightmost 2 curves, m indicates mother; c, healthy control donor. For the middle group of curves, d indicates the four affected family members. The leftmost curves consist of a second antibody alone in 3 affected family members and the healthy control donor. The same result was seen in EBV-transformed B cells (n = 5, data not shown). (D) Serum levels of sCD95 were measured in the patient, the other family members affected by the mutation (father, sister 1, and sister 2), the healthy mother (two times in each subject), and in 6 healthy control donors. Error bars indicate standard deviation.