American Society of Hematology

Figure 5.

$Figure 5. Functional correction of EBV-transformed B cells from the patient with ALPS. Correction was performed by transfection (Amaxa system) with 20 μg wt-CD95 AU1-tagged plasmid (black line). As negative control, patient cells were transfected with empty plasmid (gray line). The region M1 is set to contain 0.2% negative control cells. Apoptosis was induced by incubation with the agonistic mAb Apo1 for 20 hours. Percentages reflect the fraction of corrected cells that survived the mAb Apo1 challenge. AU1 staining is shown; 1 representative of 2 transfection experiments is given.$

Functional correction of EBV-transformed B cells from the patient with ALPS. Correction was performed by transfection (Amaxa system) with 20 μg wt-CD95 AU1-tagged plasmid (black line). As negative control, patient cells were transfected with empty plasmid (gray line). The region M1 is set to contain 0.2% negative control cells. Apoptosis was induced by incubation with the agonistic mAb Apo1 for 20 hours. Percentages reflect the fraction of corrected cells that survived the mAb Apo1 challenge. AU1 staining is shown; 1 representative of 2 transfection experiments is given.

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