Production of human sCD95 did not interfere with anti-CD95–induced apoptosis in mouse BW cells. (A) Expression of human CD95 (Fas) after transfection of mouse BW cells with 20 μg plasmids as indicated. Gray lines indicate staining with goat antimouse antibody alone; black lines, Apo1 antibody added for specific staining. (B) Secretion of human sCD95 (sFas) into the cell supernatant by BW cells 2 days after transfection. (C) Loss of transfected BW mouse cells by mAb Apo1 (▦, 200 ng/mL; ▪, 500 ng/mL) and Protein A or by Protein A alone (□). The average of 2 experiments is given.