MSCs home to lymphoid organs and the subarachnoid spaces. eGFP⁺ mMSCs are detected, at day 9 after immunization, in the spleen of treated mice by immunofluorescence as shown in panels A-B (20 ×) and, at day 40 after immunization, as detected by immunohistochemistry (brown, E) (20 ×). eGFP⁺ mMSCs are observed next to T cells as revealed by triple staining for CD3 (red), DAPI (blue), and eGFP⁺ (green) in the spleen of treated animals (D) (20 ×). eGFP⁺ mMSCs are detected also in the subarachnoid spaces of the spinal cord of MSC-treated mice by immunofluorescence (G) and immunohistochemistry (H). Scattered eGFP⁺ cells are observed by immunofluorescence inside the parenchyma just beneath the subpial layer of treated mice (J) (40 ×). Negative controls are shown in panels C, F, I, and K. Arrows indicate MSCs.