DCs do not carry BCG into the ADLNs after vaccination in the ear dermis. (A) Low-density cells recovered from ADLNs from 4 to 72 hours after BCG-egfp injection were stained with anti-CD11c and analyzed by flow cytometry. (B) ADLN sections from BCG or mock-injected mice were immunostained with anti-CD11c. At 72 hours after injection, colocalization of conventional DCs and BCG was observed on rare occasions (inset), but most of the bacilli remained localized under the ADLN capsule. Images were acquired as in Figure 2B, except that cells were labeled only with Texas red, and 10 ×/0.25 NA and 40 ×/0.75 NA (inset only) objectives were used.