Relationship of Gag-specific CD4⁺ T cells to time since infection, subset changes, and viral load. (A) Comparison of levels of Gag-specific CD4⁺ T cells in early (▦;N=7) versus late (▨;N=7) PHI subjects. Box plots show 10th, 25th, median, 75th, and 90th percentiles for each cohort. (B) Plot of CCR5⁺CD38⁺⁺⁺ CD4⁺ T cells in all PHI patients versus time since onset of symptoms. The regression line is a Lowess curve. (C) Correlation of Gag-specific CD4⁺ T cells with proportion of CCR5⁺CD38⁺⁺⁺ CD4⁺ T cells in all PHI patients. Dotted horizontal line indicates zero on the y-axis. (D) Plot of CCR5⁺CD38⁺⁺⁺ CD4⁺ T cells in all PHI patients versus plasma HIV-1 RNA viral load. The linear regression curve is shown.