Radich Figure 8 (in Druker et al). Gene expression differences between chronic and blast phase CML.

Figure 8A compares the expression of 25,000 genes between a pool of blast crisis and a pool of chronic phase CML RNA. The Y axis is the relative expression of a gene in blast phase v. chronic phase. The X axis is the intensity of each “spot.” The blue dots represent all genes that are not significantly different between chronic and blast phase. The red dots are genes up-regulated in blast compared to chronic phase (n = 347 genes), and green dots are genes down-regulated in blast compared to chronic phase (n = 203 genes). Figure 8B is a “dendrogram,” which demonstrates the clustering of genes and disease states. Blast crisis cases tend to have gene expression patterns quite similar to other blast crisis cases, but different than chronic phase cases, whose patterns are also fairly similar to one another. Note that a patient with the diagnosis of chronic phase demonstrated a blast crisis gene expression pattern. This patient relapsed early after transplantation.