Nagel Figure 7
Nagel Figure 7. (in Atweh et al). Correction of sickle cell pathology by T87Q-globin containing lentivirus-transduced BERK and SAD marrow. / Nomarski optics microscopy of red cells from mice transplanted with either (top) mock- or (bottom) T87Q-globin lentivirus-transduced BERK bone marrow cells under 5% pO2 3 months after transplantation. / Quantification of the percentage of sickle red cells from recipients of mock-transduced and T87Q-globin transduced BERK or SAD bone marrow under 5% or 13% oxygen conditions, respectively.43 Error bars indicate SE; *, P = .01; †, P = .03. / Relationship between log of reciprocal delay time of HbS polymerization and Hb concentration of red cell lysates. / Time courses of Hb polymerization in lysates were performed at various concentrations by the temperature jump method: open triangle: a mouse recipient of mock-transduced SAD marrow; close square, lysate from a mouse recipient of bT87Q-globin transduced SAD marrow; open circle, a mouse recipient of mock-transduced BERK marrow; closed circle, a mou se recipient of bT87Q-globin transduced BERK marrow. / Reprinted with permission from Pawliuk R, Westerman KA, Fabry ME, et al. Correction of sickle cell disease in transgenic mouse models by gene therapy. Science. 2000;294:2368–71.

(in Atweh et al). Correction of sickle cell pathology by T87Q-globin containing lentivirus-transduced BERK and SAD marrow.

Nomarski optics microscopy of red cells from mice transplanted with either (top) mock- or (bottom) T87Q-globin lentivirus-transduced BERK bone marrow cells under 5% pO2 3 months after transplantation.

Quantification of the percentage of sickle red cells from recipients of mock-transduced and T87Q-globin transduced BERK or SAD bone marrow under 5% or 13% oxygen conditions, respectively.43 Error bars indicate SE; *, P = .01; †, P = .03.

Relationship between log of reciprocal delay time of HbS polymerization and Hb concentration of red cell lysates.

Time courses of Hb polymerization in lysates were performed at various concentrations by the temperature jump method: open triangle: a mouse recipient of mock-transduced SAD marrow; close square, lysate from a mouse recipient of bT87Q-globin transduced SAD marrow; open circle, a mouse recipient of mock-transduced BERK marrow; closed circle, a mou se recipient of bT87Q-globin transduced BERK marrow.

Reprinted with permission from

Pawliuk R, Westerman KA, Fabry ME, et al. Correction of sickle cell disease in transgenic mouse models by gene therapy. Science. 2000;294:2368–71.

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