(in Barrett et al). In situ localization of mHa-specific cytotoxic T lymphocytes (CTLs) in the skin explant assay.

Skin sections of an HLA^A2 male individual were incubated with autologous peripheral blood mononuclear cells (PBMCs)—data not shown, or with H-Y- or HA-1-specific CTLs.

Figure 2a. H-Y-specific CTLs showing grade IV reactions.

Figure 2b. Skin sections incubated with FITC-conjugated CD8 antibodies (green) plus APC-conjugated (red) tetrameric HLA-A2-H-Y peptide complex (H-Y^A2 tetramer)—analyzed by confocal microscopy

Figure 2c. Mild histopathological changes induced by HA-1-specific CTLs.

Reprinted with permission of the Nature Publishing Group (http://www.nature.com).⁴⁴