Characteristics of the P210-induced myeloproliferative disease. (A) Peripheral blood from a mouse transduced with a control retrovirus (MigR1). Original magnification × 100. (B) Typical appearance of peripheral blood from mouse with myeloproliferative disease. Original magnification × 100. (C) Higher power view of (B). Original magnification × 1,000. (D) Splenomegaly associated with myeloproliferative disease. The diseased spleen is on top and the spleen from a MigR1 control animal is below. (E) Hematoxylin and eosin (H&E) section of spleen from (D). The red pulp is replaced by sheets of granulocytes. Original magnification × 400. (F) Hypercellular murine CML bone marrow. The great majority of cells are mature granulocytes. Original magnification × 400. (G) Liver in murine CML shows infiltration of mature myeloid cells and EMH in sinusoids. Original magnification × 100. This mouse did not have the macrophage expansion. (H) Murine CML liver with macrophage expansion (arrow). Note infiltrating hematopoietic cells in sinusoids. Original magnification × 400. (I) Pulmonary infiltrates of EMH in P210 mice. Original magnification × 100. (J) T-cell lymphoma associated with blast transformation (from an abdominal mass). Original magnification × 400. This tumor developed after 2 rounds of serial transplant of the myeloproliferative disease from mouse H2. (K) Wright-Giemsa staining of cells from peripheral blood of mouse receiving Mig210-transduced bone marrow cells. Original magnification × 400. (L) Abl expression in the cells from (K) as detected by the abl monoclonal antibody 24-11. Original magnification × 400. (M) Wright-Giemsa staining of cells from peripheral blood of mouse receiving Mig210-transduced bone marrow cells. Original magnification × 400. (N) Staining with an isotype control. Original magnification × 400.