Fig. 5.
Immunophenotypes of cells obtained from peripheral blood, spleen, bone marrow, and liver of mice receiving either P210 (Mig210) or control (MigR1) bone marrow cells. Mig210 expresses both P210 bcr/abl and GFP, whereas MigR1 expresses only GFP. GFP fluoresces in the FL1 channel. The lineage-specific antibodies (Gr1, Mac1, B220, Thy1.2, and Ter119) were directly or indirectly labeled with PE and fluoresce in the FL2 channel. Hematopoietic cells were not present in the livers of mice receiving MigR1. Ter119 staining was not performed on the cells derived from MigR1 peripheral blood. The Mig210 data were obtained from mouse BB12 and are a representative Mig210 phenotype. The MigR1 data were obtained from mouse FF4 and are a representative MigR1 phenotype.