Fig. 2.
Kinetics of anti-IgM–induced apoptosis in Ramos B cells. Cells were incubated for the varying time intervals in the presence of isotype control MOPC-21 (A through G), HB57 anti-IgM (H through N), or Mu53 anti-IgM (O through U) (10 μg/mL; MoAb:cell ratio = 2 μg/105 cells). Cultured cells were stained with FITC-annexin V and analyzed by flow cytometry as in Fig 1. The value shown in the upper right-hand corner of each histogram represents the percentage of cells that were brightly annexin-positive after each culture interval.
