American Society of Hematology

Fig. 6.

$Fig. 6. (A) RT-PCR analysis of flt3 and c-kit mRNA expression in HPCs and various lymphocyte populations. Lanes 1 through 3, purified CD34+ HPCs from 3 normal BM donors; lanes 4 and 5, CD56+CD3− NK cells generated from CD34+ HPCs after culture in FL plus IL-15 for 3 weeks; lanes 6 and 7, CD56bright blood NK cells; lanes 8 and 9, CD56dim blood NK cells from two normal blood donors; lane 10, unfractionated PBL; lane 11, blood CD3+ T cells. (Below) Human β-actin control of the identical samples. (B) Proliferation of CD56bright NK cells in response to various concentrations of IL-2, IL-2 plus FL (100 ng/mL), and IL-2 plus KL (100 ng/mL). The results are representative of three separate experiments and indicate the mean cpm ± SEM of triplicate measures.$

(A) RT-PCR analysis of flt3 and c-kit mRNA expression in HPCs and various lymphocyte populations. Lanes 1 through 3, purified CD34⁺ HPCs from 3 normal BM donors; lanes 4 and 5, CD56⁺CD3⁻ NK cells generated from CD34⁺ HPCs after culture in FL plus IL-15 for 3 weeks; lanes 6 and 7, CD56^bright blood NK cells; lanes 8 and 9, CD56^dim blood NK cells from two normal blood donors; lane 10, unfractionated PBL; lane 11, blood CD3⁺ T cells. (Below) Human β-actin control of the identical samples. (B) Proliferation of CD56^bright NK cells in response to various concentrations of IL-2, IL-2 plus FL (100 ng/mL), and IL-2 plus KL (100 ng/mL). The results are representative of three separate experiments and indicate the mean cpm ± SEM of triplicate measures.

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