Effect of incubation of RBC and inhibitors on NF-kB activity in HUVEC nuclear extracts by gel-shift assay. HUVEC were incubated with RBC (2% Hct) in the presence of E-CM (100 μg/mL) for 60 minutes, unless otherwise indicated in the presence and absence of inhibitors (genistein, 25 μg/mL; KYRGDS, 100 μmol/L; HFPA, 10 μmol/L; and probucol, 50 μmol/L). Nuclear extracts were prepared and incubated with a double-stranded ³²P-labeled NF-kB oligonucleotide probe. Where indicated, excess cold competitor oligonucleotide was added to the nuclear extracts and incubated for 10 minutes before addition of radiolabeled DNA probe. The data are representative of four independent experiments.