Effect of inhibitors on the ³²P incorporation in PECAM-1 in HUVEC on incubation with RBC. ³²P-labeled HUVEC were incubated with inhibitors either for 45 minute (GF 109203X, 20 nmol/L; SQ 22536, 2 μmol/L; calyculin A, 2 nmol/L; probucol, 50 μmol/L; or for 24 hours (GSH-EE, 0.5 mmol/L and BSO, 100 μmol/L), followed by 1-hour incubation with SS RBC (2% Hct) and E-CM. Data are expressed as mean ± SD for three different SS RBC donors, in duplicate determinations, relative to untreated HUVEC (none).