Fig. 3.
Immunoprecipitation using anti-C15. (A) Immunoprecipitation followed by Western blotting. Lane 1, Km3 cell lysate without immunoprecipitation; lane 2, unlabeled Km3 lysate immunoprecipitated with preimmune IgG; lane 3, Km3 lysate immunoprecipitated with anti-C15; lane 4, anti-C15 with no lysate. The protein samples were separated in 8% SDS-PAGE, transferred to a nitrocellulose membrane, and probed with anti-C15. (B) Immunoprecipitation of radiolabeled proteins. 35S-labeled Km3 lysate (300 μL) was precleared with preimmune IgG and incubated with 1 μg anti-C15 for 3 hours at 4°C. The immune complex was pelleted by protein A/G beads, washed, separated by 10% SDS-PAGE, and autoradiographed.
