Fig. 7.
Flow cytometric analysis of subdiploid events in BLIN-2 cells cultured in the presence or absence of BM stromal cells. BLIN-2/stromal cell cultures were gently shaken to displace BLIN-2 from the stromal cells. These stromal cell-displaced BLIN-2 cells and BLIN-2 cells in medium alone were then lysed in 0.1% Triton X-100, and the nuclei were isolated and stained with propidium iodide. The percentage of subdiploid events is listed in each histogram. This experiment is representative of six similar experiments.