EPO fails to induce c-myc expression in ▵ABD cells. Full-length or ▵ABD EPO R/ABL transfected Ba/F3 cells were factor deprived overnight and then treated with EPO for 0 to 60 minutes. Fifteen micrograms of total cellular RNA was electrophoresed in each lane and analyzed by Northern blot hybridization with a c-myc cDNA as probe. Equal loading was confirmed by visualizing 18S and 28S RNA bands on the ethidium bromide-stained gel from which the blots were made.