Fig. 3.
RT-PCR of endogenous PAR-1 after transfection with antisense constructs, demonstrating relative loss of endogenous PAR-1. Lane 1, pCDNA3 containing PAR-1 cDNA; lanes 2, 3, 4, and 5, antisense constructs A2, A12, A18, and A57, respectively; lane 6, wild-type B16; and unmarked lane 7, molecular weight markers. A12 (lane 3) was used for the in vitro and in vivo antisense experiments.
