Fig. 2.
![Fig. 2. Detection of apoptosis in CLL cells (patient UPN9a) as detected by DNA strand breaks by in situ terminal deoxynucleotidyl transferase/Propidium iodine assay (TdT/PI or tunnel) after continuous exposure to medium ([A] 4 hours and [C] 24 hours) or 0.18 μmol/L flavopiridol ([B] 4 hours and [D] 24 hours). Increasing uptake of biotinylated dUTP and avidin FITC as depicted in (B) and (D) correlates with the number of DNA fragments per cell. Cell cycle analysis is achieved by the intercalation of propidium iodine into cellular DNA.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/10/10.1182_blood.v92.10.3804/4/blod42236002cx.jpeg?Expires=1735849760&Signature=KQHu4g4PgvJy9Fi2j-1OWc-LoiqZWKd7vgRMqL~xutInvq54tEJ8S7B4-IM24~Va2DhhtI~kLzfHfHnXtbjlCp-9lS3IyKiKnvxFDzFRlK8txA6kKXyVaGPw~kupma0gIWzSQGkfh7g-iuPSTaEtkKWYgpnpzPlXs0-dt9lO21XTQh6EQ-4uoKbPNNghIYtbLL8oAEB63Vy7~slxegej9tAOZRUuhT1RNLRB-B7t0nvXfvf-bsKO~LpjIyXLeShYMwuUDyPC4pW6XRm4aMD43HTWzrLkRZyjvMhCpdvGM3d0eRrt-GoTcqI7k0m0yQGMHwtGtIEZ9hgfy2oweMG2lQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Detection of apoptosis in CLL cells (patient UPN9a) as detected by DNA strand breaks by in situ terminal deoxynucleotidyl transferase/Propidium iodine assay (TdT/PI or tunnel) after continuous exposure to medium ([A] 4 hours and [C] 24 hours) or 0.18 μmol/L flavopiridol ([B] 4 hours and [D] 24 hours). Increasing uptake of biotinylated dUTP and avidin FITC as depicted in (B) and (D) correlates with the number of DNA fragments per cell. Cell cycle analysis is achieved by the intercalation of propidium iodine into cellular DNA.
