Fig. 3.
![Fig. 3. Detection of apoptosis in CLL cells (patient UPN28a) as detected by annexin-V/PI assay after continuous exposure to medium ([A] 4 hours and [C] 24 hours) or 0.18 μmol/L flavopiridol ([B] 4 hours and [D] 24 hours). Phosphatidyl serine is restricted to the internal cell membrane but is externalized early during the process of apoptosis. Cellular uptake of propidium iodine indicates a disrupted cellular membrane generally observed during late apoptosis or with cell necrosis. Increased annexin binding to exposed phosphatidyl serine in quadrant 3 of (B) and (D) as compared with (A) and (C) is supportive of flavopiridol inducing cytotoxicity at least in part through the pathway of apoptosis in human CLL cells.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/10/10.1182_blood.v92.10.3804/4/blod42236003dx.jpeg?Expires=1735850472&Signature=GnayNoPAoA2CfWGG4fq6PMlmXDSw0gb3RyLcdfRxY110D2oMaWWhrWAldNY0uVmy60I49-ppAC8cPgSxfUTKFlZu6rH0brItQt~OZaG372I~LQHYhg67OaZnifMsNZ10Z~eyxw50Y0UYFh1svCD2ts62DXmnp~zFJ5zzL~zoUsQ4ZjRpN7neJfo03-fjPbMOvq-Ux4XMnSmshjdyur9hTxpyqlMGK6eAEVBCBc~bsN-YNnUhiWmWbjmfab5KYvdLvUJ4TVaWpfZubZLVEjAtNsPz7lo6f5EouFVAjNZ6pcsvM6wj8H4uNnQvNAZRrf4EuFAP-w8ZDzD3e6leNqC~lw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Detection of apoptosis in CLL cells (patient UPN28a) as detected by annexin-V/PI assay after continuous exposure to medium ([A] 4 hours and [C] 24 hours) or 0.18 μmol/L flavopiridol ([B] 4 hours and [D] 24 hours). Phosphatidyl serine is restricted to the internal cell membrane but is externalized early during the process of apoptosis. Cellular uptake of propidium iodine indicates a disrupted cellular membrane generally observed during late apoptosis or with cell necrosis. Increased annexin binding to exposed phosphatidyl serine in quadrant 3 of (B) and (D) as compared with (A) and (C) is supportive of flavopiridol inducing cytotoxicity at least in part through the pathway of apoptosis in human CLL cells.
