Eosinophil accumulation on IL-4–stimulated HUVECs. Confluent monolayers of primary HUVECs were washed once with HBSS and then stimulated with either M199/A alone (control) or M199/A containing 20 ng/mL IL-4 (IL-4). After 24 hours at 37°C, the monolayers were washed with HBSS and assembled in the flow chamber. Purified eosinophils (5 × 10⁵/mL) were perfused over the monolayers at the specified shear stresses and eosinophil accumulation (A), rolling, and adhesion (B) were determined. The data represent the mean and SEM of at least three independent experiments. *P ≤ .05.