Inhibition of BzATP-stimulated IL-1β release from THP-1 cells by hP2X7 receptor MoAb. THP-1 cells, pretreated with LPS for 18 hours, were incubated (150,000 per well) at 37°C for 30 minutes in the absence (•) or presence of (○) 0.012 μg/mL, (▪) 0.038 μg/mL, (□) 0.12 μg/mL, (▴) 0.38 μg/mL, or (▵) 1.2 μg/mL of the hP2X7 MoAb. BzATP was added and, after 30 minutes of incubation at 37°C, the cell suspension was centrifuged at 200g for 5 minutes and the IL1-β present in a 10-μL aliquot of supernatant was determined using a reporter assay as described in the Materials and Methods. The data are the mean ± standard error of the mean of three experiments. In each experiment, the maximal release of IL-1β was determined and the data were expressed as a percentage of this release (0% and 100% represent 0.4 ± 0.05 and 16 ± 1 ng of IL-1β per well, respectively).
