Immunophenotyping of leukemic cells from Bcr-Abl–BMT mice by flow cytometry. Two-parameter dot-plots show expression of lineage-specific antigens versus GFP as indicated. (Top panel) The expression of B220, Thy1.2, Ter119, Mac-1, and Gr-1 versus GFP, as indicated, in the peripheral WBCs from a primary Bcr-Abl–BMT mouse with the myeloproliferative disorder. (Bottom panel) The expression of Mac-1 versus GFP in the peripheral WBCs, spleen, liver, lung, and bone marrow from a secondary Bcr-Abl–BMT mouse with the myeloproliferative disorder.