Analysis of MSCV-bcr-abl/p210-IRES-gfp proviral integration in Bcr-Abl–BMT mice. The genomic DNA isolated from peripheral WBCs or tissues of the Bcr-Abl–BMT mice was analyzed by Southern blot with ³²P-labeled IRES-gfp sequences (A, B, and D) or a 1.2-kb SgrAI-Bgl II fragment from the 3′ end of the human c-abl cDNA (C) as a probe. (A) Peripheral WBCs of 5 primary Bcr-Abl–BMT (lanes 1 through 5) and 7 secondary recipient mice (lanes 6 through 12) transplanted with bone marrow cells of BMT5.3 (lane 5). (B) Peripheral WBCs of the primary Bcr-Abl–BMT mouse, BMT 5.2 (lane 1), and its secondary recipients, BMT6.43 (lanes 4 and 5) and BMT6.50 (lane 6), and the liver (lane 2) and spleen (lane 3) of BMT6.43. (C) The filter from (B) was stripped and reprobed with ³²P-labeled abl cDNA. (D) Peripheral blood, spleen, and liver of the primary Bcr-Abl–BMT mice 4.27 and 4.28. P, peripheral WBCs; P1 and P2 specify the peripheral WBCs taken from BMT6.43 in two different days; L, liver; S, spleen.