Expression of Bcr-Abl/p210 protein in the peripheral WBC from a Bcr-Abl–BMT mouse with the myeloproliferative disorder and expression of the wild-type and kinase-negative mutant Bcr-Abl/p210 proteins in NIH 3T3 cells. (A) Peripheral WBC from two Bcr-Abl–BMT mice with the myeloproliferative disorder (BMT4.18 and BMT5.8) were lysed directly in SDS sample buffer. The total cell lysates were subjected to Western blot analysis with anti-Abl monoclonal antibody Ab-3. The position of Bcr-abl/p210 and endogenous c-Abl is indicated. Total cell lysates from uninfected NIH3T3 and NIH3T3 infected with retrovirus containing the bcr-abl/p210 gene were used as negative and positive controls, respectively. (B) NIH 3T3 cells infected with equal amounts (1 mL) of MSCV-IRES-gfp (lane 1), MSCV-bcr-abl/p210-IRES-gfp (lane 2), and MSCV-K1176R-IRES-gfp (lane 3) retroviral supernatants of equal titer were subjected to Western blot analysis with anti-Abl monoclonal antibody Ab-3.