Fig. 8.
![Fig. 8. Detection of gene expression of cytokines in bone marrow of transplanted mice by RT-PCR. Bone marrow cells from Bcr-Abl–BMT mice (BMT5.1 [lanes 1 and 2], BMT5.4 [lanes 3 and 4], and BMT5.6 [lanes 5 and 6]) and a vector-BMT mouse (BMT5.35 [lanes 7 and 8]) was collected, and RNA was extracted as described in Materials and Methods. The RT-PCR products generated with (RT+) or without (RT−) reverse transcriptase were subjected to agarose gel electrophoresis, stained with ethidium bromide, and photographed by Gel Doc 1000 (Bio-Rad, Hercules, CA) with inverse gray scale. The RT-PCR products of IL-3, GM-CSF, and SCF are indicated. GAPDH was used as a control for the quality and quantity of the RNA samples and the RT-PCR reactions.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/10/10.1182_blood.v92.10.3829/4/blod42214008w.jpeg?Expires=1735547801&Signature=vFjKqsszrywtKRqc7rLUTVTEQjBDHJxAFD7L5myaQKVAVJrCuKxmHROPKudkKooSQbNFGqsgtnRh9W1kRYEcC26t3nNNM3q8rfO4u0p3nBmjkYek71su3AyKq46pioywxpYsXHXpmhcCRLR-k5ad0PpBRTkd0dWO08ePNmi~NfWUtmQ0rD-YGA6sLxLj8hc-XeBLXxRchmhZ3eEfYHfdzLe-s7Rgt0wJNzICRc2bz37-EiQJRq2jxaiJvm5HB6LANiDjRVcJZ4HNlCk3p7YzGq8dw88jgOEehVapkGDEkevEKQ4xe7vNOI8QtDxF~IqZ0jWV7BkGh9GczbvhgnvhQQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Detection of gene expression of cytokines in bone marrow of transplanted mice by RT-PCR. Bone marrow cells from Bcr-Abl–BMT mice (BMT5.1 [lanes 1 and 2], BMT5.4 [lanes 3 and 4], and BMT5.6 [lanes 5 and 6]) and a vector-BMT mouse (BMT5.35 [lanes 7 and 8]) was collected, and RNA was extracted as described in Materials and Methods. The RT-PCR products generated with (RT+) or without (RT−) reverse transcriptase were subjected to agarose gel electrophoresis, stained with ethidium bromide, and photographed by Gel Doc 1000 (Bio-Rad, Hercules, CA) with inverse gray scale. The RT-PCR products of IL-3, GM-CSF, and SCF are indicated. GAPDH was used as a control for the quality and quantity of the RNA samples and the RT-PCR reactions.
