Fig. 9.
![Fig. 9. Detection of gene expression of IL-3 and GM-CSF in GFP-positive and GFP-negative bone marrow cells from Bcr-Abl–BMT mice. Bone marrow cells from Bcr-Abl–BMT mice (BMT8.40 [lanes 1 through 4] and BMT8.41 [lanes 5 through 8]) were isolated and treated with ACK to lyse RBC. GFP-positive and GFP-negative bone marrow cells were sorted by flow cytometry and their RNA was extracted as described in Materials and Methods. The RT-PCR was performed and analyzed as in Fig8. The RT-PCR products of IL-3, GM-CSF, GFP, and GAPDH are indicated.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/10/10.1182_blood.v92.10.3829/4/blod42214009w.jpeg?Expires=1735547641&Signature=Ko8g9ycWrc~E9go8SFWhj24sytlH7KeTRjVfHIwFRjKJpDzjX1JQJFIOkXpux5g0DFjAD~PemzQesypyyO1Jsv~nug4bQRV2vNs3~Hi4TvrllYiUreA7QaEOda8uMMq8LVoP2E2Llv~RGDvbE19C5FVStS6tNF4mmP0kGZ4ei24be2frIx~lmWAGkboGorme7v1nRti25-F2CObvdg8AXFhaxB2pdIzym7oGHDqNJOPY7Cx5Fm4Lr19QYQ2bY7V7j5iHw4Df-P7jrSzX13QovRq7uU5dVso~KQ30C~D5qtQTVkYXRo2AVfHi9WxuaYMhZWYOQLK~I8SX-vE32ls6Vg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Detection of gene expression of IL-3 and GM-CSF in GFP-positive and GFP-negative bone marrow cells from Bcr-Abl–BMT mice. Bone marrow cells from Bcr-Abl–BMT mice (BMT8.40 [lanes 1 through 4] and BMT8.41 [lanes 5 through 8]) were isolated and treated with ACK to lyse RBC. GFP-positive and GFP-negative bone marrow cells were sorted by flow cytometry and their RNA was extracted as described in Materials and Methods. The RT-PCR was performed and analyzed as in Fig8. The RT-PCR products of IL-3, GM-CSF, GFP, and GAPDH are indicated.
