Proliferative response of 32Dcl3 cell clones to G-CSF or IL-3.

Using the Cell Census Plus System (Sigma) the proliferative response of clones NEO.1-NEO.3 and D-7.1-D-7.5 was measured following stimulation with either G-CSF (100 ng/mL) or IL-3 (30 ng/mL) for 72 hours. The raw data indicating the percent of cells in each generation (Gen 0-Gen 9) and the “proliferation index” (see “Materials and methods”) is shown at the right for clones stimulated with G-CSF (A) or IL-3 (B). The proliferation index for G-CSF stimulation was normalized to the proliferation index for IL-3 stimulation for each clone and this value (% IL-3 response) is presented in C.