NO-induced HEL cell apoptosis and effect of TPO.

(A) Percentage of B and C COMET produced by treatment with 10 μmol/L GSNO (NO) in the presence or absence of 100 ng/mL TPO (T), 100 μmol/L IL-11,11 or 100 μmol/L serotonin (S) pretreatment. (B) Percentage of B and C COMET produced by treatment with cytokines (C) to induce iNOS in the presence or absence of TPO IL-11 or serotonin. (C) Percentage of B and C COMET achieved by exposure to NO produced by co-culture with fibroblasts in which iNOS had been induced, in the presence of TPO, IL-11, or serotonin. In all experiments, treatment with TPO, IL-11, or serotonin in the absence of a source of NO did not lead to the production of B and C COMET (0%). Each bar represents the average of 3 experiments (*P < .05).