Expression of transgenes that contain mutant GATA sites, NF–E2 sites, or both in undifferentiated and 3-day differentiated embryoid bodies.

(A) Summary of the constructs that contained mutations in either GATA, NF–E2, or both (mGATA, mNF–E2, or mGATA/mNF–E2). The mutant constructs contain point mutations that abolish the consensus site for its transcription factor. δGATA contains a complete deletion of the GATA cis-element. All mutations were introduced into the −0.375 kb promoter fragment. (B) Either undifferentiated (D0, black bars) or 3-day differentiated (D3, hatched bars) ES cells were disrupted to single cells by brief trypsinization and analyzed for EGFP transgene expression using flow cytometry. The graph is representative of 3 independent experiments.