Binding of VT-FITC to PMNs in whole blood was demonstrated by flow cytometric analysis.

(A, B) Flow cytometric analysis of control blood (incubated with IgG-FITC alone) before addition of VT-FITC. (C, D) Analysis after 20-minute incubation with 0.5 μL VT-FITC (1 mg/mL) exclusive binding to PMNs was found. L indicates lymphocytes; M, monocytes; P, PMNs. The experiment is representative for duplicate determinations in 10 experiments with blood of different donors.