Fig. 3.
PMNs of 2 different donors were used to determine binding of 125I-VT-1 to PMNs.
(A) PMNs were incubated with increasing concentrations of125I-VT-1 (0.3 to 70 nmol/L) at 4°C for 3 hours (▴). Nonspecific binding (•) was determined in the presence of 25-fold excess of unlabeled VT-1. (B) Shows the result of Scatchard plot analysis.